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recombinant human nrg1β-1 peptide (PeproTech)

Name: recombinant human nrg1β-1 peptide
Brand: PeproTech
Rating: 90 (1 reviews)

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PeproTech recombinant human nrg1β-1 peptide
Recombinant Human Nrg1β 1 Peptide, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human nrg1β-1 peptide/product/PeproTech
Average 90 stars, based on 1 article reviews

recombinant human nrg1β-1 peptide - by Bioz Stars, 2026-03

90/100 stars

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(A) Endogenous ErbB4 in MCF7 cells was stimulated using saturating levels of NRG1β (25 nM/ 200 ng/ml) or NRG1α (1 μM/ 7 μg/ml). Activating phosphorylation of ErbB4 and ERK1/2, as well as total ErbB4 and ERK levels, were monitored by immunoblotting total cell lysates. Grb2 was used as a loading control. Blots are representative of at least three independent experiments. Statistical significance of differences was assessed using the Bonferroni-Dunn method (alpha = 0.05), with datasets for each point analyzed individually without assuming consistent SD.

Journal: Science signaling

Article Title: Kinetics of receptor tyrosine kinase activation define ERK signaling dynamics *

doi: 10.1126/scisignal.aaz5267

Figure Lengend Snippet: (A) Endogenous ErbB4 in MCF7 cells was stimulated using saturating levels of NRG1β (25 nM/ 200 ng/ml) or NRG1α (1 μM/ 7 μg/ml). Activating phosphorylation of ErbB4 and ERK1/2, as well as total ErbB4 and ERK levels, were monitored by immunoblotting total cell lysates. Grb2 was used as a loading control. Blots are representative of at least three independent experiments. Statistical significance of differences was assessed using the Bonferroni-Dunn method (alpha = 0.05), with datasets for each point analyzed individually without assuming consistent SD.

Article Snippet: Recombinant human NRG1α (Cat. #296-HR) and NRG1β (Cat. #396-HB/CF) were obtained from R&D Systems.

Techniques: Phospho-proteomics, Western Blot, Control

(a) CEM/ErbB4 cells were manually stimulated with 10 nM NRG2β (full agonist positive control), 10 nM NRG2β/Q43L (partial agonist positive control), and mock (negative control). A single batch of lysate was assayed for ErbB4 tyrosine phosphorylation in eight independent trials using a manual sandwich ELISA. (b) CEM/ErbB4 cells were stimulated in six independent trials using a semi-automated protocol with 10 nM NRG1β (positive control) and mock (negative control). Each batch of lysate was assayed for ErbB4 tyrosine phosphorylation using a manual sandwich ELISA. (c) CEM/ErbB4 cells were stimulated using a semi-automated protocol with 10 nM NRG1β (positive control) and mock (negative control). A single batch of lysate was assayed for ErbB4 tyrosine phosphorylation in three independent trials using a semi-automated sandwich ELISA.

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a) CEM/ErbB4 cells were manually stimulated with 10 nM NRG2β (full agonist positive control), 10 nM NRG2β/Q43L (partial agonist positive control), and mock (negative control). A single batch of lysate was assayed for ErbB4 tyrosine phosphorylation in eight independent trials using a manual sandwich ELISA. (b) CEM/ErbB4 cells were stimulated in six independent trials using a semi-automated protocol with 10 nM NRG1β (positive control) and mock (negative control). Each batch of lysate was assayed for ErbB4 tyrosine phosphorylation using a manual sandwich ELISA. (c) CEM/ErbB4 cells were stimulated using a semi-automated protocol with 10 nM NRG1β (positive control) and mock (negative control). A single batch of lysate was assayed for ErbB4 tyrosine phosphorylation in three independent trials using a semi-automated sandwich ELISA.

Article Snippet: Recombinant NRG1β was obtained from PeproTech (Catalog number 100–03, Lot number 0711316 and Lot number 0913316), and NRG2β isoforms and mutants have been described previously [ , , ].

Techniques: Positive Control, Negative Control, Phospho-proteomics, Sandwich ELISA

(a) BaF3/EGFR+ErbB4 cells were stimulated with increasing concentrations of NRG1β in four independent trials using a semi-automated protocol. A semi-automated MTT assay was used to analyze cell proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the EC 50 of NRG1β. The Z-factor for ErbB4-dependent cell proliferation stimulated by 0.3 nM NRG1β was calculated using the means and standard deviations of the positive (0.3 nM NRG1β) and negative (NRG1β-diluent) controls. (b) BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of gefitinib in the presence of 0.3 nM NRG1β in four independent trials using a semi-automated protocol. A semi-automated MTT assay was used to analyze cell proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 of gefitinib against NRG1β.

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a) BaF3/EGFR+ErbB4 cells were stimulated with increasing concentrations of NRG1β in four independent trials using a semi-automated protocol. A semi-automated MTT assay was used to analyze cell proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the EC 50 of NRG1β. The Z-factor for ErbB4-dependent cell proliferation stimulated by 0.3 nM NRG1β was calculated using the means and standard deviations of the positive (0.3 nM NRG1β) and negative (NRG1β-diluent) controls. (b) BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of gefitinib in the presence of 0.3 nM NRG1β in four independent trials using a semi-automated protocol. A semi-automated MTT assay was used to analyze cell proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 of gefitinib against NRG1β.

Techniques: MTT Assay

(a-d) In three independent trials and using semi-automated processes, BaF3/EGFR+ErbB4 cells were stimulated with increasing concentrations of NRG1β in the presence or absence of gefitinib at 300 nM or candidates at 30 uM. A semi-automated MTT assay was used to analyze cell proliferation at 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the EC 50 and E max of NRG1β in the presence and absence of gefitinib or the candidates. EC 50 and E max values are also shown in .

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a-d) In three independent trials and using semi-automated processes, BaF3/EGFR+ErbB4 cells were stimulated with increasing concentrations of NRG1β in the presence or absence of gefitinib at 300 nM or candidates at 30 uM. A semi-automated MTT assay was used to analyze cell proliferation at 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the EC 50 and E max of NRG1β in the presence and absence of gefitinib or the candidates. EC 50 and E max values are also shown in .

Techniques: MTT Assay

Effect of candidates on stimulation of cell proliferation by NRG1β.

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: Effect of candidates on stimulation of cell proliferation by NRG1β.

Techniques:

(a-b) In three independent trials and using a modified version of our semi-automated processes, BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of a candidate inhibitor or gefitinib (positive control inhibitor of NRG1β-induced proliferation) in the presence of 0.1 nM IL3 or 0.3 nM NRG1β. A semi-automated MTT assay was used to analyze cellular proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 value for each inhibitor against 0.1 nM IL3 and 0.3 nM NRG1β. IC 50 values are also shown in . *Extrapolated value.

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a-b) In three independent trials and using a modified version of our semi-automated processes, BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of a candidate inhibitor or gefitinib (positive control inhibitor of NRG1β-induced proliferation) in the presence of 0.1 nM IL3 or 0.3 nM NRG1β. A semi-automated MTT assay was used to analyze cellular proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 value for each inhibitor against 0.1 nM IL3 and 0.3 nM NRG1β. IC 50 values are also shown in . *Extrapolated value.

Techniques: Modification, Positive Control, MTT Assay

Effect of increasing concentrations of candidate inhibitors on stimulation of cell proliferation by 0.3 nM NRG1β or 0.1 nM IL3.

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: Effect of increasing concentrations of candidate inhibitors on stimulation of cell proliferation by 0.3 nM NRG1β or 0.1 nM IL3.

Techniques: Control

(a-e) In three independent trials and using a modified version of our semi-automated processes, BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of each candidate inhibitor in the presence of 0.1 nM IL3 or 0.3 nM NRG1β. A semi-automated MTT assay was used to analyze cellular proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 value for each candidate against 0.1 nM IL3 and 0.3 nM NRG1β. IC 50 values are also shown in .

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a-e) In three independent trials and using a modified version of our semi-automated processes, BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of each candidate inhibitor in the presence of 0.1 nM IL3 or 0.3 nM NRG1β. A semi-automated MTT assay was used to analyze cellular proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 value for each candidate against 0.1 nM IL3 and 0.3 nM NRG1β. IC 50 values are also shown in .

Techniques: Modification, MTT Assay

Journal: PLoS ONE

Article Title: Development and application of high-throughput screens for the discovery of compounds that disrupt ErbB4 signaling: Candidate cancer therapeutics

doi: 10.1371/journal.pone.0243901

Figure Lengend Snippet: (a-b) In three independent trials and using a modified version of our semi-automated processes, BaF3/EGFR+ErbB4 cells were treated with increasing concentrations of each candidate inhibitor in the presence of 0.1 nM IL3 or 0.3 nM NRG1β. A semi-automated MTT assay was used to analyze cellular proliferation 120 hours post-stimulation. Curves were fit to the data using GraphPad Prism to determine the IC 50 value for each candidate against 0.1 nM IL3 and 0.3 nM NRG1β.

Techniques: Modification, MTT Assay

(A) Isolated TNCCs plated on Boyden inserts in the presence of different molecular gradients. Data is internally normalized to control. All gradients tested except NGF were significantly different than control (p<0.05 by paired t-test; n=circles on graph/experimental dates). GDNF, glial cell-derived neurotrophic factor. Red line, 100% of control. (B) Isolated TNCC plated on FN slides were filmed with or without 200ng/ml of NRG1. Total migrated distance and Euclidean distance were normalized showing increased migration after NRG1 treatment (p<0.07 and p<0.02 respectively. N=3 experiments, N of cells =86). (C) Velocity of cells in B was measured, with NRG1 treated cells moving twice as fast as control cells (p<0.017). (D–I) NT explants cultured in Boyden chambers with an NRG1β gradient (−/+), no NRG1β (−/−), or homogenous NRG1β (+/+). (D–G) Representative HNK1 staining of chemotaxis filters showing a higher percent TNCCs on the bottom side in −/+ versus −/− chambers. Top of filter in focus (D,E); bottom in focus (F,G). Bar, 25 μm. (H) Percent HNK1-positive and percent HNK1-negative transmigrated cells. n=7 experimental dates (>33 NT explants per treatment). p<0.0001 for HNK1-positive −/− versus −/+ treatments, 1-tailed Dunnett’s. (I) Combined TNCC density from top and bottom sample areas. All t-tests described: no multiple testing adjustments made. Bar graphs show means +/− SEM.

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Neuregulin-1 is a chemoattractant and chemokinetic molecule for trunk neural crest cells

doi: 10.1002/dvdy.24625

Figure Lengend Snippet: (A) Isolated TNCCs plated on Boyden inserts in the presence of different molecular gradients. Data is internally normalized to control. All gradients tested except NGF were significantly different than control (p<0.05 by paired t-test; n=circles on graph/experimental dates). GDNF, glial cell-derived neurotrophic factor. Red line, 100% of control. (B) Isolated TNCC plated on FN slides were filmed with or without 200ng/ml of NRG1. Total migrated distance and Euclidean distance were normalized showing increased migration after NRG1 treatment (p<0.07 and p<0.02 respectively. N=3 experiments, N of cells =86). (C) Velocity of cells in B was measured, with NRG1 treated cells moving twice as fast as control cells (p<0.017). (D–I) NT explants cultured in Boyden chambers with an NRG1β gradient (−/+), no NRG1β (−/−), or homogenous NRG1β (+/+). (D–G) Representative HNK1 staining of chemotaxis filters showing a higher percent TNCCs on the bottom side in −/+ versus −/− chambers. Top of filter in focus (D,E); bottom in focus (F,G). Bar, 25 μm. (H) Percent HNK1-positive and percent HNK1-negative transmigrated cells. n=7 experimental dates (>33 NT explants per treatment). p<0.0001 for HNK1-positive −/− versus −/+ treatments, 1-tailed Dunnett’s. (I) Combined TNCC density from top and bottom sample areas. All t-tests described: no multiple testing adjustments made. Bar graphs show means +/− SEM.

Article Snippet: Amplification was done over 35 cycles (95°C 5minutes, 94°C 30 seconds, 58°C 30 seconds, 72°C 1 minute) for RT-PCR (Eppendorf master cycler gradient). table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse HNK-1 IgM DSHB Cat#1C10 Alexa 488 anti-mouse IgM Invitrogen Cat# A-21042 Mouse anti-c-erbB-4 IgG1 Thermo Scientific Cat#MS270P0 Rabbit anti-GFP IgG Life Technologies Cat# A-11122 Rhodamine Red-X anti-mouse IgM Jackson ImmunoResearch, West Grove, PA Cat#115-295-075 594 F(ab′)2 fragment of anti-mouse IgG ThermoFisher Cat#A-11020 488 anti-rabbit IgG ThermoFisher Cat#A-11034 594 anti-rabbit IgG ThermoFisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"A11037","term_id":"492397","term_text":"A11037"}} A11037 647 anti-mouse IgM ThermoFisher Cat#A-21238 Bacterial and Virus Strains pcDNA3-DNerbB4 Rio et al. 1997 pcDNA3-EGFP Addgene #13031 Biological Samples Chicken embryos (Gallus gallus) AA Laboratories, Westminster, CA Quail embryos (Coturnix coturnix) McIntyre Poultry, Lakeside CA Chemicals, Peptides, and Recombinant Proteins Children’s Oncology Group Cell Culture and Xenograft Repository http://cogcell.org/ NRG1-EGF Domains NRG1α R&D Systems Cat#296-HR-050 BSA ThermoFisher Cat#BP-1600-100 NRG1β R&D Systems Cat#396-HB-050/CF Tryphostin AG 1478 Enzo Cat #ALX-270-036-M001 BPE BD Cat# 356123 Live Cell Imaging Solution Life Technologies Cat# A14291DJ Critical Commercial Assays Boyden Chamber Assay BD Immulon 4 ELISA well Dynatech Laboratories, Chantilly, VA microchips Caltech Microfluidic Foundry www.kni.caltech.edu/foundry Modified Zigmond Chamber Assay Walheim et al., 2012 Deposited Data Experimental Models: Cell Lines Experimental Models: Organisms/Strains Oligonucleotides Recombinant DNA Software and Algorithms Excel Microsoft https://products.office.com/en-us/excel SPSS IBM https://www.ibm.com/us-en/marketplace/spss-statistics ImageJ Manual Tracking ImageJ https://imagej.nih.gov/ij/plugins/track/track.html ImageJ Chemotaxis and Migration Tool ImageJ http://ibidi.com/software/chemotaxis_and_migration_tool AxioVision Rel.

Techniques: Isolation, Control, Derivative Assay, Migration, Cell Culture, Staining, Chemotaxis Assay

A NT explant (darker tissue) was plated within a region of an ELISA well pre-coated with NRG1α and filmed for 18 h as TNCCs emigrated from the NT explant and were confronted by the coat border (red curved line). A–C: Numerous emigrating cells can be seen migrating freely outside a PBS area (arrows). D–F: Numerous emigrating cells can be seen excessively lingering near the NRG1α border and/or preferentially migrating to open spaces still within the NRG1-coated region not occupied by other cells before migrating outside the coated region (arrows).

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Neuregulin-1 is a chemoattractant and chemokinetic molecule for trunk neural crest cells

doi: 10.1002/dvdy.24625

Figure Lengend Snippet: A NT explant (darker tissue) was plated within a region of an ELISA well pre-coated with NRG1α and filmed for 18 h as TNCCs emigrated from the NT explant and were confronted by the coat border (red curved line). A–C: Numerous emigrating cells can be seen migrating freely outside a PBS area (arrows). D–F: Numerous emigrating cells can be seen excessively lingering near the NRG1α border and/or preferentially migrating to open spaces still within the NRG1-coated region not occupied by other cells before migrating outside the coated region (arrows).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Neuregulin-1 is a chemoattractant and chemokinetic molecule for trunk neural crest cells

doi: 10.1002/dvdy.24625

Figure Lengend Snippet: RT-PCR analysis

Techniques: Virus, Recombinant, Cell Culture, Live Cell Imaging, Boyden Chamber Assay, Enzyme-linked Immunosorbent Assay, Modification, Software, Chemotaxis Assay, Migration

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